关键词: 重叠蛋白质点, 角点检测, 多边形近似, 凹点, 分割

Abstract: A segmentation method of overlapping protein spots is proposed for detection of overlapping protein spots in two-dimensional gel electrophoresis images, which is based on the combination of corner detection and polygonal approximation. First, Harris corner detector is used to distinguish the corner points from the overlapping protein spots. Second, the corner points are used to make polygonal approximation of protein spots and the concave corner points are obtained by judging the convexity and concavity of polygonal vertices. Finally, overlapping protein spots are separated by constructing the segmentation lines while selecting the concave points from the concave corner points under the concave points selecting and matching rule. Experimental results show that the method can be used to separate the overlapping protein spots accurately, especially the severe overlapping protein spots.

Key words: overlapping protein spots, corner detection, polygon approximation, concave point , segmentation