Abstract: Three-dimensional fluorescence spectrometry is a new fluorescence analysis technology.Because of the high computational complexity of algorithms，in the process of development of new portable devices based on three-dimensional fluorescence spectrometry，the structure design of computing-intensive component becomes the key point.This paper gives a run-time analysis to the common three-linear analysis algorithms and discusses the hardware design and implementation method of SVD component.The result shows that，the design method of SVD hardware component based on iterative QRD has a better area efficiency with some acceptable speed sacrifice，and is more appropriate for three-dimensional fluorescence spectrometry analysis algorithms.