Abstract: When the environment is under high-speed，there will be a large Doppler because of time-vary channel.So，Channel State Information（CSI） must be estimated at any time.Aiming at this problem，the total least squares is introduced to estimate CSI.Considering channel noise and the character of time-varying of channel，this algorithm can estimate not only received signal but also channel state.At the same time，it can track them，too.In time-varying channel，TLS method has the advantages of lower complexity and fast convergence.Compared with LS and ML algorithms，TLS has better BER performance.The simulation results show that TLS method is effective in channel estimate in wireless communication system.