Abstract: Bioinformatics is a body of science which solve biological problems using methodology from computer science. It mainly includes how to storage, retrieval and analyze bioinformation by employing computer. With the startup and implementation of Human Genome Project, nucleic acid and protein data has been increased rapidly. It is an urgent problem that how to gain useful information from plentiful data. There is a good combination between data mining and bioinformatics. The potential application of data mining in bioinformatics has been given more and more attention. The paper introduced the basic concepts of bioinformatics and data mining, and reviewed several typical applications of data mining in bioinformatics.