关键词: p53家族基因, 基因签名, 核苷酸游离电子的平均能量（EIIP）, 层次聚类分析

Abstract: On the basis of original gene signature method with biological characteristics, the introduction of the average energy of delocalized electrons（EIIP） of the nucleotide with certain physical characteristics, establishes a new E-gene signature method. At the same time, an E-Euclidean distance and an e-average variance formula of two sequences are defined and the related species are analyzed by hierarchical cluster analysis. The results are obtained by conducting E-gene signature on the p53 gene family mRNA of the complete CDS of 20 species, both in each of the genes, the relationship between species closer, the similarity of gene signature higher. In addition, the clustering results with the original clustering results of gene signature method for comparison and find that the E-gene signature method is more accurate, thus it is more conducive to in-depth understanding of the nature of the p53 gene family.

Key words: p53 gene family, gene signature, Electron-Ion Interaction Pseudopotential（EIIP）, hierarchical clustering analysis