Abstract: Because of its advantages in speed and cost, Serial transmission technology becomes a preferred choice and has been widely used in the high-speed communication field. Most of the traditional high-speed serial transmission interfaces are built based on the Aurora link level protocol. However, the protocol itself could cause a waste of resource to some extent. Thus, we present a new solution on the design of high-speed serial transmission interface, which can achieve a 2.5Gbps data transmission rate by defining a new serial transmission protocol and using high-speed serial transceiver 'Rocket I/O'.